Skip to main content
. 2013 Jul 23;110(32):E2977–E2986. doi: 10.1073/pnas.1301021110

Fig. 5.

Fig. 5.

AID knockdown blocks up-regulation of EMT markers in MCF10A cells. (A) AID (AICDA) mRNA levels measured by real-time qPCR, displayed relative to levels in MCF10A parental cells expressing a scrambled shRNA (shScr). MCF10A cells expressing shAIDkd1 display an approximate fivefold reduced level of AID mRNA. *P < 0.001. (B) mRNA levels of key EMT factors in MC10A cells deficient for AID expression (shAIDkd1) in EMT-inducing conditions, determined by real-time qPCR and displayed as fold change relative to MCF10A shScr cells. P < 0.05 Vim, P < 5 × 10−4 CDH2, P < 1 × 10−4 SNAI1, P < 0.005 HMGA2, and P < 5 × 10−6 MMP2 and MMP9. Error bars indicate SD (n ≥ 3 experiments).