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. 2013 Jul 1;110(32):12948–12953. doi: 10.1073/pnas.1304386110

Fig. 1.

Fig. 1.

Nucleotide-dependence of GlnK–AmtB complex formation. Complex formation between His6-AmtB and GlnK in the presence of various effectors was assessed by elution from a His-Select Spin column. The effectors present for each lane are indicated in the table below the figure. Preincubation indicates that GlnK was preincubated with the effectors for 15 min at 30 °C before loading onto the column.