Fig. 3.

Cohesin dissociation from DNA does not trigger Smc3 deactylation. Release of cohesin from chromatin via Scc1 (B) but not Smc3 cleavage trigger de-acetylation (A). Strains K20018, K20019, K20021, and K20022 were grown in medium lacking tryptophan [2% (wt/vol) glucose] and then transferred in YEP medium containing 2% raffinose for 5 h. Subsequently, cells were arrested in metaphase by nocodazole for 2 h and then 2% galactose was added to induce the expression of TEV. Western blots against the indicated proteins were performed on whole-cell extracts. Full pictures of A are shown in SI Appendix, Fig S12C. An asterisk indicates a-specific bands.