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. 2013 Jul 22;110(32):12911–12916. doi: 10.1073/pnas.1222041110

Fig. 5.

Fig. 5.

Evaluation of sulfatase inhibitors. Mycobacterial lysates (10 μg) were incubated for 1 h with phosphatase inhibitor mixture (lane 1), arylsulfamate (lanes 2–5), metal chelator (lanes 6–7), or water (lane 8). Lysates were resolved by electrophoresis before incubation with 10 μM DDAO-sulfate in 100 mM Tris⋅Cl (pH 7.5 at 37 °C) with 100 mM NaCl. Lane 1, phosphatase inhibitor mixture; lane 2, coumarin sulfamate (Stx64); lane 3, phenylsulfamate; lane 4, cyanosulfamate; lane 5, chlorosulfamate; lane 6, tetrakis-(2-pyridylmethyl)ethylenediamine; lane 7, 1,10-phenanthroline monohydrate; lane 8, water.