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Kinetic parameters for substrate dose responses at saturating calcium
| Substrate | no IgG | Control IgG | RA27-P4 IgG | RA45-XR IgG |
|---|---|---|---|---|
| Histone H3 | ||||
| Km (M) | 2.56 × 10−7 | NA | 3.73 × 10−7 | 3.44 × 10−7 |
| kcat (s−1) | 6.05 × 10−3 | NA | 2.36 × 10−3 | 8.88 × 10−3 |
| kcat/Km (M−1s−1) | 23600 | NA | 6330 | 25800 |
| BAEE | ||||
| Km (M) | 0.96 × 10−3 | 1.13 × 10−3 | 1.36 × 10−3 | 0.25 × 10−3 |
| kcat (s−1) | 0.242 | 0.271 | 0.415 | 0.113 |
| kcat/Km (M−1s−1) | 252 | 241 | 306 | 448 |
NA= not assayed
