Figure 5.

IL-6 upregulates the expression of anti-apoptotic proteins. A, CHLA-255 cells were treated with IL-6 (10ng/mL) and cells were lysed at indicated time points (hours). Total cell lysates (20µg/lane) were then examined by Western blot for the expression of indicated anti-apoptotic proteins. Actin was used as loading control. The data are representative of 3 separate experiments showing similar results. The bar graph represents the fold increase compared to control after the ratios of anti-apoptotic protein:actin were normalized for the ratio at 0 hour. B, CHLA-255 cells were treated with IL-6 and slL-6R and examined for survivin expression by immunofluorescence. Stattic (2.5 mM) was added 4 hours before IL-6 and sIL-6R when indicated (scale bar = 20µm). C, siRNA transfected CHLA-255 cells treated as above described were examined for pSTAT3, survivin and Bcl-xL expression by Western blot after 24 hours of stimulation with IL-6 and sIL-6R. The data are representative of 3 experiments showing similar results.