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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Microbiol. 2013 Jun 17;89(2):350–371. doi: 10.1111/mmi.12281

Figure 11.

Figure 11

Holdfasts restored due to suppression by overexpression of holdfast export proteins are anchored to the cell body. Comparison of holdfast restored in (A) ΔpodJ and (B) ΔhfsB mutants by holdfast export protein overexpression. Holdfast material from whole cell lysates was detected on a nitrocellulose membrane using chemiluminescence from an HRP conjugated lectin (top rows). In the blots on the bottom rows, the amount of holdfast in cell culture supernatants is detected in a volume equal to 15 times that of the whole cell culture sample. Culture and corresponding supernatant samples were taken from the same culture and were from the same blot. Each set of samples (cell culture with corresponding culture supernatant for ΔpodJ or ΔhfsB) is representative of several independent blots, but is from the same blot. (C) ΔpodJ and ΔhfsB mutants in which holdfast export proteins were overexpressed remain attached to a glass slide after thorough washing with water.