Abundance of holdfast export proteins during the cell cycle. Samples were taken every 15 min during a time course. The resulting Western blot was probed with α-HfsD, α-HfsA, α-HfsB, and α-McpA antibodies. The chemoreceptor protein McpA, which undergoes cell-cycle regulated proteolysis, was used as a synchrony control. The approximate developmental stage of the cell cycle is depicted above.