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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Microbiol. 2013 Jun 17;89(2):350–371. doi: 10.1111/mmi.12281

Figure 6.

Figure 6

A small proportion of ΔpodJ cells can synthesize holdfast whereas holdfast is never detected in a ΔpleC mutant. (A) Left panel: Total amount of holdfast in a whole culture as detected using a dot blot. Representative spots were cropped from the same blot. Middle panel: The percentage of individual planktonic cells that bind fluorescent WGA was quantified and normalized to wild-type as a reference strain. The mean and its standard error are shown. Cells (200 to 500 per sample) were counted from four independent cultures and then averaged after normalization. Right panel: The percentage of total polarly localized holdfasts was calculated. The mean and its standard error are shown. (B) Developmental mutants were tested using the small volume cell attachment assay and fluorescent holdfast labeling. DIC and fluorescent image overlays that are representative of the density of cells remaining attached to a glass slide after washing are shown. Additional panels can be found in Figure S4.