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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Microbiol. 2013 Jun 17;89(2):350–371. doi: 10.1111/mmi.12281

Figure 8.

Figure 8

Overexpressing HfsD in holdfast synthesis mutants and ΔpleC does not restore holdfast synthesis. (A) Total holdfast in whole cultures of the developmental mutant ΔpleC overexpressing HfsD (top row) and two required synthesis glycosyl transferase mutants (ΔhfsE ΔpssY ΔpssZ and ΔhfsG) overexpressing HfsD (bottom row) was measured by dot blotting with HRP-WGA. Each sample set was cropped from a different blot (three total). (B) Overexpressing HfsD in a pleC podJ double mutant does not restore holdfast. Cells from these strains do not bind fluorescent lectin. DIC and fluorescence image overlays are shown for each strain.