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Asymmetry of holdfast inheritance is perturbed in cells that produce mis-localized holdfast. ΔpodJ/pUJ142hfsD cells were observed using time-lapse microscopy (see Movie S2). A pre-divisional cell is shown in the first panel. After cell division, both daughter cells will inherit holdfast. Top row: DIC images overlaid with fluorescence. Bottom row: fluorescence signal from AlexaFluor 488-conjugated WGA.
