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Kinetic Parameters for Group V (phosphoryl amidates) hydrolysis by rePON1 G2E6
| Molecule ID |
 |
kcat s−1 |
KM mM |
kcat/KM M−1 s−1 |
IC50 mM |
|---|---|---|---|---|---|
| R | |||||
| 34 |  |
ND | ND | ND | 0.1–0.5 |
| 35 |  |
ND | ND | ND | 0.5–1 |
| 36 |  |
∼ 0.3 a | ND | > 300 a | 0.03 |
ND Not determined.
Due to substrate inhibition at higher concentrations, we could only estimate kcat and a kcat/KM (see text and Supporting Information).
