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Circular dichroism spectra of wild-type nitrate reductase and its K58Q and K130Q variants. Spectra were measured at ambient temperature in a 1.0 cm optical pathlength cell using solutions of either wild-type nitrate reductase or the indicated variant. All samples contained nitrate reductase at a concentration of 0.75 μM in 10 mM potassium phosphate buffer (pH 7.5).
