Table 2.

Primers used to introduce mutation in the pCSLM85 nitrate reductase expression plasmid.

Primer Name	Sequence (5′-3′)
K58Q-For	GGAATGGTCTGCGTCCAGGGCGCAACGGTGGCG
K58Q-Rev	CGCCACCGTTGCGCCCTGGACGCAGACCATTCC
K58R-For	GGAATGGTCTGCGTCAGAGGCGCAACGGTGGCG
K58R-Rev	CGCCACCGTTGCGCCTCTGACGCAGACCATTCC
R70Q-For	TCTGTCAGCAAAAGCCAGCTGAAATATCCAATG
R70Q-Rev	CATTGGATATTTCAGCTGGCTTTTGCTGACAGA
R70K-For	TCTGTCAGCAAAAGCAAGCTGAAATATCCAATG
R70K-Rev	CATTGGATATTTCAGCTTGCTTTTGCTGACAGA
K130Q-For	TACTACATCGCCCAACAGCTGGTGAAAGGTTGC
K130Q-Rev	GCAACCTTTCACCAGCTGTTGGGCGATGTAGTA
K130R-For	TACTACATCGCCCAACGGCTGGTGAAAGGTTGC
K130R-Rev	GCAACCTTTCACCAGCCGTTGGGCGATGTAGTA
K146Q-For	TTCGATACCAACTCGCAGCTCTGCATGTCCTCA
K146Q-Rev	TGAGCACATGCAGAGCTGCGAGTTGGTATCGAA