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Elevated FUS1-lacZ reporter gene activity in constitutively signaling mutant strains. β-galactosidase activity resulting from the FUS1-lacZ pheromone-responsive reporter gene is shown for cells carrying the indicated STE2 gene on either a low copy YCp plasmid or a high copy YEp plasmid. Overproduction of the STE2-P258L mutant results in higher β-galactosidase activity because it compensates for the reduced stability of this mutant receptor at the cell surface.
