Skip to main content
. Author manuscript; available in PMC: 2013 Aug 12.
Published in final edited form as: Methods Enzymol. 2010;485:329–348. doi: 10.1016/B978-0-12-381296-4.00019-1

Figure 5.

Figure 5

Dominant-negative STE2 mutants are resistant to α-factor-induced cell division arrest. Yeast strain JKY25 carrying a wild-type copy of STE2 in the genome were transformed with either low copy or high copy plasmids carrying the STE2 genes indicated on the left. For simplicity, the empty vector control was labeled as ste2Δ. Equal amounts of cells were then spotted on medium lacking α-factor, or containing 10−8 or 10−7 M α-factor as indicated above. Growth of cells was photographed after incubation for 2 days at 30°C. [Reproduced from (Dosil et al. 1998) with permission from the American Society for Microbiology (DOI = 0270-7306/98/$04.00+0)].