Table 2.
Concordance, sensitivity, specificity, and accuracy of Sanger sequencing, real-time polymerase chain reaction with TheraScreen® kit, pyrosequencing, and chip array infiniti® assay measured using 454 next-generation sequencing as the reference method
| Method | Kappa of concordance | Sensitivity | Specificity | Accuracy |
|---|---|---|---|---|
| Sanger sequencing | 0.791 | 34/40 (85%) | 100% | 54/60 (90%) |
| TheraScreen (ΔCt) | 0.857 | 36/40 (90%) | 100% | 56/60 (93%) |
| Pyrosequencing (percent mutated) | 0.892 | 37/40 (93%) | 100% | 57/60 (95%) |
| Chip array assay (ratio)* | 0.891 | 36/39 (92%) | 100% | 56/59 (95%) |
Note:
*
One sample not assessed, values calculated on 59/60 cases.