Figure 1. Effects of MTX with or without fish oil (FO) and/or genistein (Gen) supplementation on growth plate, primary and secondary spongiosa.

Paraffin sections of the tibial metaphysis region (PS =  Primary spongiosa, SS =  Secondary spongiosa, which are separated by a dashed line) of (A) a normal rat, (B) a MTX+H₂O treated rat showing reduced height of primary spongiosa and. metaphyseal bone volume, (C) a MTX+FO treated rat, and (D) a MTX+Gen treated rat. (E) Growth plate total height (µm). (F) Primary spongiosa height (µm). (G) Secondary spongiosa BV/TV (%). (H) Secondary spongiosa osteoblast/mm² trabecular bone area. Values are means ± SEM; n = 7–8 for all groups. Labelled means without a common letter differ (P<0.05).