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. 2013 Aug 12;8(8):e71592. doi: 10.1371/journal.pone.0071592

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© 2013 Raghu Nadhanan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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H&E-stained sections of tibial lower secondary spongiosa in a (A) control rat and (B) a MTX alone treated rat. (C) Adipocyte numbers on bone histology sections. (D) Nile Red-stained images of cultures showing adipocyte formation in an ex vivo adipogenesis assay with bone marrow stromal cells of a control rat and (E) a MTX+H₂O treated rat. (F) Quantification of Nile Red⁺ colonies in an ex vivo adipogenesis assay from bone marrow cells of treated rats. RT-PCR relative gene expression analysis of adipogenesis related genes (G) PPARγ and (H) FABP4 assessed in the isolated bone marrow stromal cells. Labelled means without a common letter differ (P<0.05).