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. 2013 Aug 12;8(8):e71743. doi: 10.1371/journal.pone.0071743

Table 2. Immunohistochemical protocols – essential data.

Primary antibody Clone Dilution Link Specificity
Anti-lepto Polyclonal 1∶5.000 Envision System/AP DAKO Leptospiral antigens
TTF1 Monoclonal 1∶500 Ultravision LP, Value Detection system,Lab Vision Corporation Pneumocytes type II and Clara cells
CD34 cod. NCL-END Monoclonal 1∶500 NOVOCASTRA cod. NCL - EWO Glycoproteins of the endothelial cells membrane
Alpha ENaC Novus Biologicals cod NR p1 20097 Polyclonal 1∶700 Ultravision LP, Value Detection system,Lab Vision Corporation Epithelial sodium channel
Anti-Connexin 43 C6219 Polyclonal 1∶400–1∶700 Ultravision LP,Value Detection System.Lab Vision Corporation Gap Junction Protein
Aquaporin 1 ab 9566 Monoclonal 1∶1000 Ultravision LP,Value Detection System,Lab Vision Corporation Water channels in humans endothelial cells
Aquaporin 1 ab 11023 Monoclonal 1∶20.000 Ultravision LP, Value Detection System,Lab Vision Corporation Water channels in human endothelial cells
Aquaporin 5 ab78486 Polyclonal 1∶150 Ultravision LP, Value detection System,Lab Vision Corporation Water channels in pneumocytes type I