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. 2013 Aug 12;8(8):e71441. doi: 10.1371/journal.pone.0071441

Figure 6. Effect of forskolin and SQ22536 on collagen secretion and mRNA expression in HSFs.

Figure 6

(A) Treatment of cultured human scleral fibroblasts (HSFs) for 24 h with forskolin significantly decreased the total soluble collagen recovered in the culture medium in a concentration-dependent manner (*: p<0.05, **: p<0.01, ANOVA, n = 6; ▴: p<0.01, ANOVA, n = 6). (B) Treatment with forskolin for 24 h significantly decreased the expression of mRNAs for collagens I, III, and V. Forskolin reduced the mRNA level in a concentration-dependent manner. (*: p<0.05, **: p<0.01, ANOVA, n = 6). (C) The reductions in collagen I, III, and V mRNA expression levels by forskolin were significantly decreased by SQ22536 treatment. (**: p<0.01, independent sample t-test, n = 6; ▴: p<0.05, independent sample t-test, n = 6).