Table 1. Sequencing statistics for sliced single-stage wild-type mRNA-Seq samples.
| Replicate | Slice | Carrier Species | Barcode Index | Total Reads | Uniquely mapped D. mel reads (%) | Ambiguous Reads (%) |
| 1 | 1 | D. per | 1 | 69,339,972 | 2,284,228 (3.2%) | 1,634,055 (2.3%) |
| 1 | 2 | D. per | 2 | 73,632,862 | 3,706,630 (5.0%) | 1,603,444 (2.1%) |
| 1 | 3 | D. per | 3 | 82,076,328 | 6,002,034 (7.3%) | 1,774,485 (2.1%) |
| 1 | 4 | D. per | 4 | 73,437,708 | 6,401,565 (8.7%) | 1,592,665 (2.1%) |
| 1 | 5 | D. per | 5 | 75,922,812 | 4,951,178 (6.5%) | 1,559,097 (2.0%) |
| 1 | 6 | D. per | 6 | 78,623,784 | 1,355,079 (1.7%) | 1,574,067 (2.0%) |
| 2 | 1 | D. wil | 7 | 59,813,036 | 4,066,295 (6.7%) | 878,476 (1.4%) |
| 2 | 2 | D. wil | 8 | 90,961,338 | 15,212,716 (16.7%) | 1,301,095 (1.4%) |
| 2 | 3 | D. wil | 9 | 73,201,902 | 14,855,374 (20.2%) | 911,768 (1.2%) |
| 2 | 4 | D. wil | 10 | 75,754,772 | 23,858,301 (31.4%) | 1,136,031 (1.4%) |
| 2 | 5 | D. wil | 11 | 84,497,566 | 10,026,713 (11.8%) | 1,080,910 (1.2%) |
| 2 | 6 | D. wil | 12 | 66,316,952 | 13,122,508 (19.7%) | 898,776 (1.3%) |
| 3 | 1 | D. moj | 13 | 75,847,986 | 12,496,248 (16.4%) | 3,615,452 (4.7%) |
| 3 | 2 | D. moj | 14 | 72,497,660 | 4,005,714 (5.5%) | 803,381 (1.1%) |
| 3 | 3 | D. moj | 15 | 77,532,368 | 11,138,154 (14.3%) | 772,446 (0.9%) |
| 3 | 4 | D. moj | 16 | 83,400,882 | 8,227,562 (9.8%) | 861,839 (1.0%) |
| 3 | 5 | D. moj | 18 | 83,608,454 | 2,630,069 (3.1%) | 795,169 (0.9%) |
| 3 | 6 | D. moj | 19 | 85,823,784 | 2,239,493 (2.6%) | 829,382 (0.9%) |
Counts are for read ends. Discordant read ends are always classed as ambiguous, but failure of one end to map does not disqualify the other.