Figure 5. Respiration was decreased and ROS production was increased in the isolated mitochondria of Pgam2 mice.

(A) Oxygen consumption in isolated mitochondria was measured using an oxygen electrode cuvette. Total oxygen consumption was lower in Pgam2 mice. Values are the mean ± SEM. *p<0.05 versus NTg mice (NTg: n = 3; Pgam2 mice: n = 5). (B) Generation of H₂O₂ by isolated mitochondria. Malate and pyruvate, or succinate, were used as substrates. The generation of H₂O₂ increased in Pgam2 mice both with malate + pyruvate and with succinate. Values are the mean ± SEM. *p<0.05 versus NTg mice (n = 6 for each group). A.U.: arbitrary unit.