Figure 1. Characterization of the antibodies against mono-, di- and tri-methyl lysine. (A) Dot blot analysis of the pan anti-methyl lysine antibodies. Peptides with un- (Kun), mono- (Kme1), di- (Kme2) and tri- (Kme3) methyl lysine were blotted onto nitrocellulose membranes and probed with the antibodies, respectively. (B) Immunoprecipitation (IP) analysis of lysates prepared from HeLa cells using the antibodies. Lanes A, B and C are input, IP eluates with normal rabbit IgG (negative control) and IP eluates with anti-methyl lysine antibodies, respectively. The concentration of SDS-PAGE was 15%. (C) Lysine methylation profiles in 13 human cell lines. The protein lysates of 13 cell lines were separated by 12.5% SDS-PAGE, transferred to PVDF membranes and probed with the antibodies, respectively.