Figure 2. HLCS interacts with both MeCP2 and DNMT1 in vivo, judged by co-immunoprecipitation assay. Myc-HLCS and HA-DNMT1 were overexpressed in HEK293 cells and protein extracts were immunoprecipitated using anti-Myc (A) and anti-HA (B). Proteins were resolved by electrophoresis and probed with anti-DNMT1 (A) and anti-HLCS ( B).Panels C and D are similar to A and B. But HA-MeCP2 was substituted for HA-DNMT1 in overexpression experiments and anti-MeCP2 (C) and anti-Myc (D) were substituted for anti-DNMT1 and anti-HLCS, respectively. Transfections with empty vectors were used as negative controls (second lane in each gel), and whole cell lysates were used as input controls (third and fourth lane in each gel). Gels were electronically re-arranged to facilitate comparisons.