Figure 1. (A) Agarose gel electrophoresis of equivalent amounts of genomic DNA from 3 d old embryos, larvae, pupae and adults digested using: Hin6I restriction enzyme (lanes: 1-embryos with plasmid pUC18R as a control of digestion, 2-embryos, 3-larvae, 4-pupae, 5 -adults). (B) Methylation-sensitive restriction enzyme HpaII (lanes: 2-embryos, 5-adults) and with the methylation-insensitive restriction enzyme MspI (lanes: 3-embryos, 6- adults). Undigested DNA from embryos and adults is present in lanes 1 and 4, respectively. (C) Methylation specific GlaI enzyme digestion of DNAs from embryos (lane 2) and from adults (lane 4) compared with the undigested controls (lanes: 1-embryos, 3-adults). (D)PvuRts1I enzyme digestion of DNA from embryos, larvae, pupae and adults (lanes 1, 2, 3, 4 respectively) compared with the undigested controls (ND). The tracks labeled L contain GeneRuler Ladder mix (Fermentas).