Figure 5. Electropherograms of a part of TCAST satellite sequence after bisulfite treatment of genomic DNAs isolated from adults (A), from adults heat shocked for 3 h (B) and 20 h (C). Red and blue peaks indicate thymine and cytosine, respectively. Each cytosine position is indicated by yellow column and a single position within TCAST region where the cytosine is present in completely unmethylated state is marked and underlined. For each sample, the methylation status of polyubiquitin and cinnabar gene promoters were also checked and the results were identical for all samples, as shown in (D) and (E).