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. 2013 Aug 12;8(8):e70623. doi: 10.1371/journal.pone.0070623

Figure 2. Intracellular localization of the SLAC1 protein in tobacco BY-2 cells.

Figure 2

Confocal fluorescence images (a-f) of BY-2 protoplast expressing SLAC1-GFP (a–c) or GFP (d–f) stained with the fluorescent styryl membrane probe FM4-64. Fluorescence of GFP (a and d) and FM4-64 (b and e). Scale bar: 10 µm. FM4-64 was kept as a 17 mM stock solution in sterile water, and used at a final concentration of 4.25 µM. Tobacco BY-2 protoplasts were treated with FM4-64 for 10 min and washed twice with the wash buffer at room temperature to label the PM.