Figure 5. miR200c through modulation of growth factor signaling through cSrc activation promotes fibrosis.

Human liver fibroblasts transfected with miR200c or scrambled miRNA were stimulated with TGF-β and analyzed for the expression of: a) cSrc at the message level by quantitative RT-PCR and b) relative expression levels of cSrc and FGF at protein level, c) representative western blot analysis of cSrc, and d) representative western blot analysis of FGF. miR200c overexpression results in a significant increase in expression of cSrc at the message level (2.6 fold) and cSrc and FGF at the protein level (3.1 and 4.2 fold respectively). Bars represent the mean relative expression levels from 3 different experiments performed with 3 different fibroblasts.