Figure 4. CigR shows anti-virulence activity.

129SvJ mice were infected IP with wild-type, ΔcigR, and a complemented derivative of this strain expressing cigR from a low coy plasmid. For each bacterial strain, five 129SvJ mice were infected at a dose of 10⁴ CFU/mouse and monitored for 14 days. The percentages of surviving mice are shown here for each strain. Differences in survival were statistically analyzed with a Kaplan-Meier test (p<0.0001). Mice infected with ΔcigR bacteria succumbed to infection, whereas mice infected with the wild-type or a complemented strain did not die.