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. 2013 Aug 12;8(8):e71064. doi: 10.1371/journal.pone.0071064

Figure 5. Quantification of cell proliferation in zebrafish retina exposed to MNU.

Figure 5

A. Before MNU exposure (day 0) few PCNA-positive cells were found in the ciliary marginal zone (CMZ) and the outer nuclear layer (ONL). Additionally, in the MNU 50 mg/l group, few PCNA-positive cells were observed in the inner nuclear layer (INL) at day 8, whereas at all other time points, no relevant signs of proliferation were found. Ganglion cells (GC) revealed no relevant cell proliferation. B. In the 150 mg/l group, PCNA-positive cells were seen in the INL starting at day 3, and their number was highest at day 8 (p≤0.0001). Proliferating cells in the ONL were not found before day 5, but their number was highest at day 15 (p≤0.0001). From day 30 on, almost no proliferating cells were observed in the INL, whereas proliferation in the ONL occurred until the end of the follow up at day 90 Cell counts refer to retinal sections of 450 μm length. Mean values with SEM error bars are represented (* indicates p≤0.0001 compared with day 0).