Figure 3. Phylogenetic tree of members of the phylum Chlamydiae, including epitheliocystis agents, calculated using NJ and MP analysis of the full length 16S rRNA gene sequence (1477 bp), using Ricksettia sp. as an outgroup.

The percentage of replicate trees in which the associated taxa cluster together in the bootstrap test (1000 replicates) is indicated. Edited sequences were aligned in ClustalW2. Bootstrap values of 85%, and 43% in the NJ and MP trees, respectively, separated the new species from its closest relatives in the Simkaniaceae family.