Skip to main content
. 2013 Aug 12;8(8):e71087. doi: 10.1371/journal.pone.0071087

Figure 6. H&E staining results of the cerebral cortex and hippocampus.

Figure 6

(A) The hippocampal CA1 area (arrows) and cortical region above the hippocampus (dashed box) were used for analysis (magnification, 400×). (B) The histogram demonstrates the density of normal neurons in the cerebral cortex and hippocampus CA1 area. One-way ANOVA followed by LSD post hoc test showed no significant differences in terms of densities of normal neurons in the cerebral cortex between the Al and MF+Al groups as well as between the control and MF groups. Quantitative results of hippocampal neurons were similar to those of the cerebral cortex. Differences between the control and MF groups as well as between the Al and MF+Al groups were not significant. The same statistical analysis results were obtained by using Kruskal-Wallis H test followed by the Mann-Whitney U test. **P<0.01 vs. control group, ## P<0.01 vs. MF group, n = 6 per group. (C) Control group and (E) MF group: normal morphology with few shrunken neurons. (D) Al group and (F) MF+Al group: normal neurons with distinguishable cell membranes and clear nucleoli close to shrunken (arrows) and swollen (arrowheads) neurons. Bar = 200 µm in A. Bar = 10 µm in C, D, E, F, n = 48 per group.