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. 2013 Aug 12;8(8):e70819. doi: 10.1371/journal.pone.0070819

Figure 4. PfCSP expression in transgenic P. berghei.

Figure 4

(A) Confocal laser scanning microscopy PfCSP expression in transgenic P. berghei. Salivary glands from the infected mosquitoes as well as transgenic P. berghei sporozoites were probed with the 2A10 mAb conjugated to Alexa Flour 594 (red). Parasite nuclei and mosquito salivary glands were visualized by DAPI (blue). Bar  = 50 µm. (B) Salivary glands from uninfected mosquitoes or mosquitoes infected with the transgenic parasites were lysed and loaded onto 10% gels, and then immunoblotted with the 2A10 mAb and anti-AAPP mAb (28B8).