FIG. 2.

Mosquito protein kinase G (PKG) phosphorylates a canonical PKG substrate. Full-length human PKGIβ (HuPKG), A. aegypti PKG (AePKG), and empty vector (EV) were expressed by a transcription and translation (TNT) reaction. A 100-μg amount of total protein from the TNT reactions was used in an in vitro CycLex PKG activity assay. Phosphorylation of a canonical PKG substrate was measured to determine the relative levels of PKG activity in each sample. Significant p values between EV and PKG samples are indicated as follows: (*) p<0.05. Samples were measured in duplicate; the figure is representative of two experimental replications.