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. 2013 Aug;33(8):452–458. doi: 10.1089/jir.2012.0083

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 1. — Expression of PE_PGRS17 in recombinant Mycobacterium smegmatis revealed by Western Blotting. The 15% sodium dodecyl sulfate–polyacrylamide gel electrophoresis exhibited a 36-kDa protein, the sum of a 31-kDa expected protein, and 5-kDa MYC tag expressed by recombinant M. smegmatis strains induced by ɛ-caprolactam. Lane 1: the protein of PE_PGRS 17 expressed in recombinant M. smegmatis strains induced by ɛ-caprolactam for 24 h. Lane 2: recombinant M. smegmatis strains containing the vector only.