FIG. 3.

Death of U937 macrophages infected with recombinant M. smegmatis. U937 macrophages were infected with Msmeg-pNIT (MYC)-Rv0978c (black bars) and M. smeg-pNIT (MYC) (white bars) at an MOI of 10, respectively. At 6-, 24-, and 48-h postinfection, culture supernatants were harvested. The release of LDH was estimated by testing its activity in the culture supernatants. The results showed that recombinant M. smegmatis containing PE_PGRS 17 increased U937 macrophages death at the MOI 10:1 in a time-dependant manner. Each treatment was repeated three times.