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. 2013 Sep;28(7):565–571. doi: 10.1089/cbr.2012.1453

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 2. — Secreted frizzled-related protein 1 (sFRP1) restoration suppressed the Wnt pathway. (A) The expression of sFRP1, GSK3β, β-catenin, cyclinD-1, and c-myc was determined by Western blotting in the A549 cell line induced by TGF-β1 (10 ng/mL). (B) The expression of GSK3β, β-catenin, cyclinD-1, and c-myc was determined by Western blotting in A549 cell line stably transfected with sFRP1 treated with TGF-β1 (10 ng/mL). (C) Luciferase reporter assays in the A549 cell line cotransfected with sFRP1 and pTopflash. **p<0.01 versus pcDNA+Topflash, ^&&pcDNA+Topflash+S33Y.