FIG. 1.
Candidate miRNAs targeting MEPE. (A) Predicted miRNAs targeting MEPE by TargetScan. (B) MEPE 3′UTR luciferase reporters. 3′ WT, wild-type 3′UTR. Mutant UTR (4 mutant sites in 3′UTR of MEPE). (C) 293T cells were co-transfected with MEPE 3′UTR luciferase reporters, along with expression plasmids of miRNAs and control vector. Renilla luciferase activity from the same construct were used to normalize and to generate relative activity of Firefly luciferase that was subject to the regulation of cloned Mepe 3′UTR. Relative luciferase level=(Sluc/Srenilla)/(Cluc/Crenilla). Luc, raw Firefly luciferase activity; Renilla, internal transfection control renilla activity; S, sample; C, control. The error bar represents the standard deviation (SD) from three independent experiments.