FIG. 2.

Downregulation of MEPE by miR-376a is mediated by 3′UTR complementary sites. (A) MEPE protein levels in HeLa cells are significantly suppressed by miR-376a. PCNA was used as protein loading control. Protein was quantified by Image J. (B) 293T cells were co-transfected with Mepe 3′UTR or mutant 3′UTR (4 of 8 bases were mutated in complementary sites) luciferase reporters, along with expression plasmids of miR-376a and control vector. Student's t-test compared two data sets marked by stars in the panel. *p<0.05, **p<0.01.