Figure 1.

Characterization of the neurogenic niche at 15 days postnatal development. (A) Scheme of a sagittal section of rat brain, highlighting the anterior ventricular zone, the neurogenic niche of the subventricular zone (SVZ), and the rostral migratory stream (RMS). Insets 1 and 2: Immunohistochemical analysis with anti-PCNA (1:100) in the SVZ and the RMS, respectively. (B) Sagittal cut of rat brain stained with hematoxylin and eosin showing the RMS (arrows). (C–J) Neurogenic niche of the brain at 15 days postnatal development analyzed with anti-PCNA, anti-S100a, anti-GFAP, and βIII-tubulin. (K–S) Immunofluorescence and confocal microscopy analyses using antibodies to identify glial and neural cells in different states of proliferation. (K–M) Brain tissue analyzed with anti-βIII-tubulin and GFAP. (N–P) Co-localization of S100a-positive ependymal cells and BrdU-positive cells. (Q–S) Co-localization of GFAP-positive glial cells and BrdU-positive cells. The images are representative of three independent experiments. CP, choroid plexus; LV, lateral ventricle; OB, olfactory bulb. Scale bars in (B), 250 μm; (C–J), 100 μm; (K–S), 50 μm.