Figure 4.

Distribution of SVCT2, GFAP, and βIII-tubulin in the cells of the ventricular area and the RMS. Immunofluorescence and confocal microscopy analyses of the ventricular neurogenic niche after 21 days postnatal development using antibodies to identify glial cells, neurons and SVCT2. (A) Sagittal section of rat brain analyzed with anti-SVCT2 and anti-βIII-tubulin. (B) Sagittal section of rat brain analyzed with anti-SVCT2 and anti-GFAP. (C–P) Co-localization of glial and neural cells with anti-SVCT2 (green), anti-GFAP (blue), and anti-βIII-tubulin in the dorsal (C–F) and ventral neurogenic niche (G–J). (K–N) Co-localization of glial and neural cells in the RMS. The images are representative of three independent experiments. Ep, ependymal cells. LV, lateral ventricle. Scale bars in (A–B), 100 μm; (C–N), 20 μm.