Fig. 3.

Density of the AchE-reactive neurons in hippocampal CA1 and CA3 of the experimental groups. AchE positive nerve fibers in the hippocampal CA1 (A) and CA3 (B) molecular layer of experimental rats in AMPK-DN+saline group (a, d), AMPK-DN+scopolamine group (b, e), and AMPK-WT+scopolamine group (c, f). The brains were cut into 40 µm coronal sections and the scale bar represents 50 µm (100×magnification). The percentage of AMPK-DN+saline values of the density of acetylcholinesterase (AChE) stained nuclei in the hippocampal CA1 (C) and CA3 (D) areas after the water maze test. Data were analyzed using one-way ANOVA followed by the Tukey's test. Each value represents the mean±SEM. ^*p<0.05 compared to AMPK-DN+scopolamine.