Fig. 5.

The effect of AMPK on glutamate-induced apopotosis of cells. Hippocampal neurons cultured in vitro for seven days were transfected with Ad-AMPK-WT and Ad-AMPK-DN in each group and then exposed to 100 µM glutamate with 10 µM of glycine in supplemented neuronal culture medium for 15 min at 37℃. Western blot assays were then conducted using anti-Bax and anti-Bcl-2. The experiment was repeated three times with similar results.