Figure 2.

The requirement for dJNK in glial engulfment of axonal debris is adult- and ensheathing-glia-specific. (a) Control animals (OR85e::mCD8::GFP, tub-Gal80^ts/+ repo-Gal4/+) and bsk^RNAi animals (OR85e::mCD8::GFP, tub-Gal80^ts/UAS-bsk^RNAi104569; repo-Gal4/+) were raised at 18 °C. After eclosion, adults were shifted to 29 °C for at least 7 days, maxillary palps were ablated, and flies were kept at 29 °C for 5 days before assaying axon clearance. (b) Quantification for (a). Error bars depict mean±S.E.M. ***P<0.001. (c) Control animals (OR85e::mCD8::GFP/+ mz0709-Gal4/+) or animals with ensheathing glia-specific knockdown of dJNK (OR85e::mCD8::GFP/UAS-bsk^RNAi104569; mz0709-Gal4/+) were axotomized and assayed for axon clearance 5 days after axotomy. (d) Quantification for (c). Error bars depict mean±S.E.M. ****P<0.0001