Figure 3.

Glial c-Jun kinase signaling to the nucleus after axotomy involves Slpr, Tak, MKK4, Jra, and Kay. (a) UAS-RNAi constructs for the indicated genes were driven in glia using the repo-Gal4 driver, axons were severed in adults, and axonal debris was scored 5 days after axotomy as above. See Supplementary Figure 3 for quantifications. (b) Slpr, tak double mutants (slpr^BS06, tak1/Y; OR85e::mCD8::GFP/+) were assayed for defects in axon clearance. (c) Model of dJNK signaling to the nucleus to induce engulfment gene expression. (d) Expression of the dAP-1 transcriptional reporter TRE-eGFP in uninjured animals (top) and animals where third antennal segments were ablated 1 day prior (bottom)