Figure 4.

dJNK is required for Draper upregulation after axotomy and activation of glial phagocytic function. (a) Immunostains for Draper in control or glial dJNK^RNAi animals before or after antennal ablation. Arrows highlight upregulation of Draper in ensheathing glia (control) or lack thereof (bsk^RNAi). (b) Draper (red) localization along the maxillary nerve (arrows) before or 1 day after maxillary palp ablation. (c) Draper immunostains (red) in a single antennal lobe before or after axotomy. A subset of maxillary palp ORN axons are shown (green). Note the lack of Draper staining within the glomerulus of glial dJNK^RNAi animals compared with controls (arrows). (d) Lysotracker Red stains for lysosomal activity one day after maxillary palp ablation. Note the punctate accumulation of Lysotracker Red in the OR85e+ glomerulus (green, white dotted circle) in controls but not in glial dJNK^RNAi animals