Figure 7.

Average apoptosis levels scored in various genetic combinations. (a) In the double mutants syp-2;msh-5, apoptosis is reduced at the same level of syp-2, spo-11, and syp-2;cep-1 double mutants, suggesting that DNA damage-induced apoptosis, but not apoptosis due to synapsis defects, is msh-5 dependent. Number of observed gonads: syp-2: 91, syp-2;msh-5: 59, syp-2;cep-1: 53, syp-2;spo-11: 58. (b) In the double mutants rad-51;msh-4, apoptosis remains at very high levels, as observed in rad-51 single mutants. Number of observed gonads: msh-4: 74, rad-51: 41, rad-51;msh-4: 42. Error bars correspond to standard error of the means (S.E.M.) calculated from at least three independent experiments. Student's t-tests are shown in Supplementary Table S1