Figure 8.

(a) Average apoptosis levels scored in mutants of the pro-CO factors zhp-3 and cosa-1 before and 24 h after irradiation with IR at 60 and 120 Gy. zhp-3 mutants do not respond to genotoxic insult as the apoptosis levels remain unperturbed at different doses of IR, suggesting that the ZHP-3 protein is required for apoptosis execution along with MSH-4/5. COSA-1, on the other hand, is not required for execution of apoptosis in pachytene cells after DNA damage. Number of observed gonads: wt 0 Gy:185, cep-1 0 Gy: 155, zhp-3 0 Gy: 149, cosa-1 0 Gy: 74, wt 60 Gy: 156, cep-1 60 Gy: 185, zhp-3 60 Gy: 139, cosa-1 60 Gy: 48, wt 120 Gy: 153, cep-1 120 Gy: 198, zhp-3 120 Gy: 110, cosa-1 120 Gy: 48. Error bars correspond to standard error of the means (S.E.M.) calculated from at least three independent experiments. Student's t-tests are shown in Supplementary Table S1. (b) Analysis of the expression of the Pegl-1::GFP reporter in a zhp-3-mutant background under physiological conditions and upon exposure to IRs. The zhp-3 mutants are competent for the induction of the Pegl-1::GFP reporter after IR in the premeiotic tip and in late pachytene cells. Scale bar 15 μm