Figure 2.

BCL-2 family protein expression as a function of Spi-1 level of expression. Expression of antiapoptotic (a) and proapoptotic (b–d) BCL-2 family members was determined by immunoblotting using lysates from shSpi-1-A2B, shSpi-1-A2C and control cells cultured with or without dox for 2 days. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) or Actin were used as a loading control. The apparent molecular weights (kDa) are indicated on the right of the panels. When indicated, cells were treated with STS